Total GIP ELISA

Measurement range: 2.7 - 1000 pmol/L

ü  Superior sensitivity

ü  Broad measuring range

ü  Measures both active GIP(1-42) and the inactive isoform GIP(3-42) in total

Description

The Mercodia Total GIP ELISA provides a method for the quantitative determination of total GIP in human samples.

Mercodia Total GIP ELISA measures active GIP(1-42) to the same extent as its metabolite GIP(3-42). Thus, the rapid degradation of the active GIP(1-42) form is not a problem for the specificity of the assay. Accurate results are obtained even if samples without any stabilizing additives are analyzed. The Mercodia Total GIP ELISA has also no cross-reactivity to known cross-reactants such as GLP-1, Glucagon, Oxyntomodulin, and Glicentin.

Want to read more about our Total GIP ELISA, see our Characterization of Total GIP NL-ELISA Reagents Report.

Test characteristics

Species：Human

Sample types：Serum and EDTA plasma

Sample volume：25µL

Assay range：2.7 - 1000 pmol/L

Detection limit：≤1.62 pmol/L

Incubation：18-22 h (overnight) + 10 min + 15 min

Detection method：Chemiluminescence

specificity

The following cross-reactions have been tested:

GIP (1-42)：114-124%

GIP (3-42)：100%

GIP (1-30)：Not detected

GIP (3-30)：Not detected

Glucagon：Not detected

GLP-1 (9-36) amide：Not detected

GLP-2：Not detected

Oxyntomodulin：Not detected

Glicentin：Not detected

Mini-glucagon：Not detected

Proglucagon 1-61：Not detected

MPGF：Not detected

PACAP-38：Not detected