Glucagon ELISA – 10 μL

Measurement range: 2-180 pmol/L (7 - 627 pg/mL)

l  Low sample volume - 10 µL

l  No purification of samples

l  Highly appreciated and often cited assay

l  Controls available: 10-1286-01

l  Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP

Description

Mercodia Glucagon ELISA – 10 μL

A revolutionizing ELISA for sensitive and specific determination of glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates (NHP). A research tool advancement that allows science to take the next step.

The highly specific monoclonal antibodies used in Glucagon ELISA – 10 µL enable accurate determination of glucagon with low to no levels of significant cross-reactivity to other circulating pro-glucagon-derived peptides. Under certain conditions like post-bariatric surgery, the concentrations of glicentin have been demonstrated to be elevated to levels that might interfere.

Mercodia Glucagon Control – Low, Medium, High (10-1286-01) is a three-level control for Mercodia Glucagon ELISA -10 µL.

An alternative protocol (TN34-0162) for Glucagon ELISA – 10 µL can be used in situations where exceptionally high levels of glicentin are expected.

Test characteristics

Species：Rat, mouse and non-human primates (NHP)

Sample types：Serum, EDTA plasma and cell culture medium

Sample volume：10 µL

Assay range：2-180 pmol/L (7 - 627 pg/mL)

Detection limit：≤1.5 pmol/L

Incubation：18-22 h (overnight) + 15 min

Detection method：Absorbance

specificity

The following cross-reactions have been tested:

Mini-glucagon：Not detected

Glicentin, human：1.0 %

Glicentin, mouse：7.0 %

Glicentin, rat：4.0 %

GLP-1 (7-36)：Not detected

GLP-1 (9-36)：Not detected

GLP-2：Not detected

Oxyntomodulin, human/rat/mouse：2.0 %

Oxyntomodulin, bovine/canine/porcine：Not detected