Ultrasensitive Mouse Insulin ELISA

Measurement range: 0.025 - 1.5 μg/L

l  Very low detection limit - 0.025 µg/L

l  High specificity - Insignificant cross-reactivity to C-peptide and proinsulin

l  No interference from complex sample matrices

l  Controls available: 10-1220-01

Description

Mercodia Ultrasensitive Mouse Insulin ELISA

A method for the quantitative determination of low levels of insulin in mouse samples. The Ultrasensitive Mouse Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia Ultrasensitive Mouse Insulin ELISA.

Test characteristics

Species：Mouse

Samples types：Serum, EDTA plasma and cell culture medium.

Sample volume：25 µL

Assay range：0.025 - 1.0 μg/L

Detection limit：≤0.025 µg/L

Incubation：2 h + 30 min

Detetction method：Absorbance

specificity

The following cross-reactions have been tested:

Rat insulin：146%

Rat proinsulin：14%

Rat C-peptide：Not detected

Human insulin：195%

Human proinsulin：82%

Human C-peptide：Not detected