Porcine C-Peptide ELISA

Measurement range: 20-1200 pmol/L

l  No cross reactivity to human proinsulin or insulin

l  No cross reactivity to C-peptide in human, macaque, rat or mouse

l  High specificity - enables specific measurement of porcine C-peptide in xenotransplantation studies

Description

The Porcine C-peptide ELISA is an assay for the quantitative determination of porcine C-peptide in serum, plasma and cell culture medium.

The Porcine C-peptide ELISA may be used for post-transplant assessment of porcine islet xenograft function in human, macaque, rat or mouse. Enables distinguishing endogenously produced C-peptide for pharmacodynamic and/or pharmacokinetic studies during exogenous insulin administration

Test characteristics

Species：Porcine

Sample types：Serum, plasma and cell culture medium

Sample volume：10 µL

Assay range：20-1200 pmol/L

Detection limit：≤10 pmol/L

Incubation：2 h + 1 h + 15 min

Detection method：Absorbance

specificity

The following cross-reactions have been tested:

Porcine insulin：<0.05 %

Porcine proinsulin：<0.02 %

Human C-peptide：<0.003 %

Human insulin：<0.002 %

Human proinsulin：<0.01 %

Macaque C-peptide：<0.006 %

Mouse insulin：<0.13 %

Mouse proinsulin：<0.002 %

Rat C-peptide：0.08 %

Rat insulin：<0.002 %

Rat proinsulin：<0.002 %