Lumi-Phos HRP ELISA HRP Substrate

Lumi-Phos HRP (PS-atto) is a proprietary acridan based chemiluminescent substrate for ELISA detection of horseradish peroxidase (HRP) conjugated molecules.Lumi-Phos HRP couples a broad dynamic range with rapid generation of sustained luminescence

l  Excellent sensitivity – less than 5 pg/mL of target protein in plate based ELISA

l  Rapid peak intensity – reduced assay time and increased throughput

l Sustained luminescence – timing is less critical

l Temperature insensitive – analytical results areminsensitive to temperatures from 22°C - 35°C reducing the need for precise temperature control

Reaction Mechanism

Excellent Sensitivity

Figure 1. Lumi-Phos HRP couples a broad dynamic range with rapid generation of sustained luminescence and superior storage stability to provide unmatched convenience to the user.

Rapid Peak Intensity

Figure 2. Lumi-Phos HRP reaches a sustained maximum light-output peak intensity within seconds of substrate addition. Signal intensities can be read at any time along the profile to produce linear calibration curves.

Product Specifications

Ordering Information