Glicentin ELISA

Measurement range: 3 – 300 pmol/L (24 – 2400 pg/mL)

ü  Highly specific, sensitive and rapid method for determination of glicentin 

ü  No cross-reactivity to circulating proglucagon derived gut peptides

ü  Assay buffers and calibrators have been optimized to prevent degradation and aggregation of glicentin

Description

The Mercodia Glicentin ELISA is a high-quality immunoassay for the quantification of human glicentin in serum, plasma, and cell culture media.

Glicentin is a proglucagon-derived gut peptide that is released in the gut in response to feed intake. It is present in high levels in the circulation after stimulation and is often a problem when measuring glucagon and other proglucagon derivates because of its shared sequence. The Mercodia Glicentin ELISA however, has no cross-reactivity to such circulating proglucagon-derived gut peptides.

Want to read more about our Glicentin ELISA and other Mercodia ELISAs, see this White Paper.

Test characteristics

Species：Human

Sample types：Serum, EDTA plasma and cell culture media

Sample volume：25 µL

Assay range：3 - 300 pmol/L (24-2400 pg/mL)

Detection limit：≤3 pmol/L (24 pg/mL)

Incubation：1 h + 1 h + 15 min

Detection method：Absorbance

Specificity

The following cross-reactions have been tested:

Glucagon：Not detected

Oxyntomodulin：Not detected

Mini-glucagon：Not detected

GLP-1：Not detected

GLP-2：Not detected

Rat glicentin：0.9 %

Mouse glicentin：Not detected