Glucagon ELISA

Measurement range: 1.5 – 130 pmol/L (5 – 453 pg/mL)

l  No cross-reactivity to glicentin, proglucagon 1-16, and glucagon 3-29

l  CE/IVD labeled on selected markets

l  No purification of samples

l  Controls available: 10-1286-01

Description

The Mercodia Glucagon ELISA is a CE/IVD-labelled, highly specific and sensitive immunoassay for measuring glucagon in plasma and serum.

The highly specific monoclonal antibodies used in this assay enable accurate determination of glucagon without significant cross-reactivity to other circulating pro-glucagon-derived peptides, e.g. glicentin.

As of October 1st, 2020, the assay protocol of Mercodia Glucagon ELISA 10-1271-01 is updated to make the sequential protocol default, abolishing cross-reactivity to glicentin. To run the old, simultaneous protocol, please refer to Technical Note TN34-161.

Mercodia Glucagon Control – Low, Medium, High (10-1286-01) is a three-level control for Mercodia Glucagon ELISA.

Test characteristics

Species：Human

Sample volume：25 µL

Sample types：Serum, EDTA plasma and cell culture medium

Assay range：1.5-130 pmol/L (5.95-453pg/mL)

Detection limit：≤1.0 pmol/L

Incubation：18-22 h (overnight) + 1 h + 30 min

Detection method：Absorbance

Specificity

The following cross-reactions have been tested:

Glicentin：Not detected

Oxyntomodulin  4.0 %

Proglucagon (1-61)  Not detected

Glucagon (3-29)  Not detected