Insulin MBeads Assay

Measurement range: 6.73 - 960 mU/L

l  Low sample volume (10µL)

l  Short and easy protocol, no dilutions

l  Adapted for manual or automatic procedures

l  If required, a 96 well bio-magnetic plate can be purchased, requesting item 15-1000-01

Description

The Insulin Northern Lights® MBeads Assay: Highly specific insulin measurements from minimal sample volumes

The Insulin MBeads Assay is a high-throughput tool designed for highly specific insulin measurement from minimal sample volumes. It is optimized for detecting human insulin in serum, conditioned media, cell lysates, and homogenates from perifusion experiments and other in vitro cultures derived from human cells.

The Insulin MBeads Assay offers high accuracy by specifically measuring human insulin while minimizing cross-reactivity with other similar cross-reactants. This assay requires only 10 µl of sample per well, reducing the need for unnecessary dilutions – ideal for volume-limited experiments.

Test characteristics

SPECIES：Human

SAMPLE TYPES：Cell media (perifusion samples, conditioned media, cell lysates and homogenates) and serum

SAMPLE VOLUME：10 μL

LLOQ：6.15 mU/L

ASSAY RANGE：6.73 - 960 mU/L

INCUBATION TIME：2 h + 5 min

DETECTION METHOD：Chemiluminescence

specificity

The following cross-reactions have been tested:

C-peptide：N.D.

Proinsulin：N.D.

Rat Insulin：N.D.

Mouse Insulin：N.D.