Iso-Insulin ELISA

Measurement range: 3 - 100 mU/L (0.13 - 4.35 µg/L)

l  Measures total insulin

l  Cross-reactivity with most insulin analogs

l  Calibrated against 1st International Reference Preparation 66/304 for human insulin.

l  CE/IVD labeled on selected markets

l  Serum controls available: 10-1134-01 (US only) or 10-1164-01

Description

Mercodia Iso-Insulin ELISA provides a method for the quantitative determination of total insulin.

A high quality immunoassay for the quantification of insulin; both endogenous and exogenous. The assay measures common, as well as non-commercially available, insulin analogues and comes with full cross-reactivity data.

Mercodia Diabetes Antigen Control (10-1134-01 (US only) or 10-1164-01) is designed to be used as a two-level control for human insulin.

Test characteristics

Species：Human

Samples：Serum, plasma, cell culture medium

Sample volume：25 µL

Assay range：3 - 100 mU/L (0.13 - 4.35 µg/L)

Detection limit：≤ 2 mU/L (0.086 µg/L)

Incubation：1 h + 15 min

Detection method：Absorbance

specificity

The following cross-reactions have been tested:

Insulin：100 %

C-peptide：<0.1 %

Proinsulin：54 %

Proinsulin des (31-32)：58 %

Proinsulin split (32-33)：56 %

Proinsulin des (64-65)：66 %

Proinsulin split (65-66)：78 %

Insulin lispro：112 %

Insulin aspart：100 %

Insulin detemir：28 %

Insulin glargine：58 %

Insulin glulisine：123 %

IGF-I：< 0.02 %

IGF-II：< 0.02 %

Rat insulin：71 %

Mouse insulin：49 %

Porcine insulin：76 %

Ovine insulin：131 %

Bovine insulin：58 %