Ultrasensitive C-peptide ELISA

Measurement range: 5 - 280 pmol/L (0.015 – 0.85 ng/mL)

ü  Very low detection limit < 2.5 pmol/L (0.0076 µg/L)

ü  No or minimal cross-reactivity to insulin or proinsulin

ü  High specificity enables measurement of human C-peptide in mouse and rat models

ü  CE/IVD labeled on select markets

ü  Serum controls available: 10-1134-01 (US only) or 10-1164-01

Description

The Ultrasensitive Mercodia C-peptide ELISA is a high-quality immunoassay for specific quantification of low levels of human C-peptide in serum, plasma, cell culture media, or urine.

The Ultrasensitive Mercodia C-peptide ELISA is a highly specific tool registered for in vitro diagnostics (IVD) use. It is calibrated against the International Reference Reagent for human C-peptide, IRR84/510. The specificity of the utilized antibodies enables measurement of human C-peptide without interference from insulin or proinsulin, and measurement of human C-peptide in mouse and rat models.

Each Mercodia C-peptide ELISA kit contains reagents for 96 wells, sufficient for 42 samples and one calibrator curve in duplicate. The microplate strip format (12 strips x 8 wells) is ideal when only a limited number of samples need to be analyzed.

Higher sensitivity can be achieved by using an alternative protocol described in technical note TN34-0144.

Mercodia Diabetes Antigen Control (10-1134-01 (US only) or 10-1164-01) is designed to be used as a two-level control in the Ultrasensitive C-peptide ELISA.

Test characteristics

Species  Human

Samples  Serum, EDTA and heparin plasma or urine

Sample volume  50 µL

Assay range  5 - 280 pmol/L (0.015 – 0.85 ng/mL)

Detection limit  ≤2.5 pmol/L (0.0076 µg/L)

Incubation  1h + 1h + 30 min

Detection method  Absorbance

Specificity

The following cross-reactions have been tested:

Insulin  < 0.0006 %

Proinsulin  5 %

Proinsulin (Des 31-32)   3 %

Proinsulin (Split 32-33)  2 %

Proinsulin (Des 64-65)  74 %

Proinsulin (Split 65-66)  10 %