Ultrasensitive Insulin ELISA

Measurement range: 0.15 - 20 mU/L (0.0065 - 0.87 µg/L) 

l  Calibrated against 1st International Reference Preparation 66/304 for human insulin

l  No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)

l  Insignificant or no cross-reactivity to insulin analogs

l  CE/IVD labeled on selected markets

l  Serum controls available: 10-1134-01 (US only) or 10-1164-01

Description

The Ultrasensitive Insulin ELISA is a high quality immunoassay for specific quantification of low levels of human insulin in serum or plasma.

The Mercodia Ultrasensitive Insulin ELISA has little or no cross-reactivity to insulin analogs, rat or mouse insulin, which enables distinguishing endogenous human insulin, both in patients undergoing treatment with insulin analogs, and in rat or mouse models.

Mercodia Diabetes Antigen Control (10-1134-01 (US only) or 10-1164-01) is designed to be used as a two-level control in the Ultrasensitive Insulin ELISA.

Test characteristics

Species：Human

Samples：Serum, plasma

Sample volume：25 µL

Assay range：0.15 - 20 mU/L (0.0065 - 0.87 µg/L)

Detection limit：≤ 0.15 mU/L (0.0065 µg/L)

Incubation：1 h + 30 min

Detection method：Absorbance

specificity

The following cross-reactions have been tested:

C-peptide：<0.01 %

Proinsulin：<0.01 %

Proinsulin des (31-32)：<0.5 %

Proinsulin split (32-33)：<0.5 %

Proinsulin des (64-65)：98 %

Proinsulin split (65-66)：56 %

Insulin aspart：3 %

Insulin detemir：Not detected

Insulin glargine：12 %

Insulin glulisine：Not detected

Insulin lispro：Not detected

IGF-I：<0.02 %

IGF-II：<0.02 %

Rat insulin：0.7 %

Mouse insulin：0.3 %

Porcine insulin：93 %

Ovine insulin：48 %

Bovine insulin：31 %